Precision single-nucleotide engineering — amino acid substitutions, codon changes, promoter tuning
| Supported Strains | P. putida KT2440, P. fluorescens DSM50090 and P. syringae DSM40610 (extended service, +2 wks) |
| SNP Types | Missense mutations, nonsense mutations, synonymous (silent) codon changes, promoter mutations (−10/−35 box), start codon changes (GTG→ATG), ribosome binding site modifications |
| Verification | Sanger sequencing of target locus + colony PCR + whole-genome sequencing available |
| Deliverable | Sequence-verified mutant strain + raw Sanger traces + strain passport |
| Turnaround | 8-12 weeks |
| Pricing | Available on request — contact us for a personalised quote |